Life Sciences & Biotechnology
Title : | Identifying modifiers of genetic diseases for preventive and prognostic interventions: focus on hematological and neurological diseases (GATII, Genomics Assisted target Identification and Intervention |
Area of research : | Life Sciences & Biotechnology, Medical Sciences |
Focus area : | Genetic Diseases |
Principal Investigator : | Sivaprakash Ramalingam, Scientist, CSIR-Institute of Genomics & Integrative Biology (CSIR-IGIB), New Delhi |
Timeline Start Year : | 2020 |
Timeline End Year : | 2023 |
Contact info : | sivaramalingam@igib. res. In |
Details
Executive Summary : | Objective: To identify of potential fetal hemoglobin associated genetic variants/modifiers of sickle cell disease. To Identify the modifier loci that interact with genetic variation linked to neurological disease such as SCAs and neuro Wilson's disease. To design, synthesis and validate sgRNAs targeting potential genetic modifiers. To engineer suitable cell types with genetic modifier(s) using CRISPR/Cas9 approach and investigate the functional associations of potential modifiers. To test the association between genetic variations in the movement related genes with the susceptibility, age of onset, and clinical features of WO in the Indian context. Generation of two to four Neuro Wilson's diseases patient-specific induced Pluripotent stem cell, from peripheral blood mononuclear cell. Differentiation of the two Neuro WD iPSC to neurons and diseases modeling of neuro WO. |
Organizations involved
