Technologies

Pharmaceutical Sciences, Chemical Sciences

Title:	Cell Penetrating Peptide, IMT-P8 as a Drug Delivery Vehicle in Management of MRSA and other Gram Positive Topical/Skin/Wound Infections
Area:	Pharmaceutical Sciences, Chemical Sciences
Focus Area:	Management of MRSA
Social Benefits:	The salient features of this technology are: The proof of concept is ready , Improved the susceptibility of several other topical antibiotics mainly used in the treatment of Gram positive bacterial infections , Higher efficiency than TAT , No cytotoxicity Non-hemolytic , Human origin, Small size (15 AA) and water soluble , In vitro and in vivo studies showed promising results, Can be a CPP-based drug formulation to tackle skin infection and wound healing at affordable cost
Developing Agency:	CSIR-Institute of Microbial Technology (IMT), Chandigarh
Technology Readiness Index:	Proof of Concept
Email:	director@imtech.res.in
Website Link :	http://www.imtech.res.in
Source (more info) :	https://t.ly/l_sb

Brief Description

Description :

Bacterial skin infections is one of the serious problems and if it is not treated at appropriate time, spreads secondary infections which is sometimes more fatal. To avoid such situations, effective and fast acting formulation is needed which is possible by combination therapy with currently used FDA approved antibiotics. Most of the antibiotics do not absorb rapidly due to their hydrophobic nature and these are obsolete. To make the use of all such antibiotics in better fashion, such options are important.There is an unmet medical need for novel topical formulations for safe and efficacious delivery of biological therapeutics across the skin leading to achieve better long-term outcomes with fewer side effects. The formulation which can deliver antibacterial across the skin more effectively than available therapy is a goal of this technology. Development of formulation, quality control parameters and determination of effective dose in approved animal model will be the component of this technology. Novel cell-penetrating peptide IMT-P8 was identified and characterized using integratedin silicoand experimental approach. IMT-P8 internalizes into a variety of human cancer and normal cells efficiently by endocytosis in a dose dependent manner without causing significant damage to cells. The biggest advantage with IMT-P8 is that it is derived from human protein and it is non-hemolytic in nature. IMT-P8 can deliver various macromolecules like proteins (GFP), nucleic acid (plasmid DNA) and small molecule fluorophores into mammalian cells. In addition, one of the promising feature of IMT-P8 is that it is also capable of penetrating the outermost dead layer of skin, i.e., stratum corneum, and it can enter into the viable epidermis and dermis after topical application. IMT-P8 can also deliver protein and small peptide into the skin layers. These features makes IMT-P8 a suitable skin penetration enhancer for various topical/transdermal formulations for the topical delivery of macromolecules like therapeutic proteins which cannot internalize into the skin by their own.