Life Sciences & Biotechnology
Title : | To explore the prevalence and contribution of duplicate genes in rewiring the regulatory network following the whole genome triplication in crop plant cauliflower |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Prabhakaran soundararajan, National Institute Of Plant Genome Research (NIPGR), New Delhi |
Timeline Start Year : | 2024 |
Timeline End Year : | 2027 |
Contact info : | prabhu@nipgr.ac.in |
Equipments : | server |
Details
Executive Summary : | The growth and development process in Brassica plants requires the coordination of hundreds of thousands of genes, involving multi-complex gene convolution to complete tasks with lesser energy requirement. This system becomes more complicated due to additional whole-genome triplication after diverging from the Arabidopsis lineage. stem cells maintained in the shoot apical meristem (sAM) act as a reservoir for generating above-ground architecture. WUsCHEL (WUs) - CLAVATA (CLV) play a central role in prolieferation and differentiation of stem cells. WUs synthesis in the organizing center (OC) and stabilized by high cytokinin (CK) move towards the central zone (CZ) as homodimers via plasmodesmata. After the decline of CK outside the OC, WUs concentration starts to decrease and becomes monomer. During flower development, indeterminated sAM groups are converted to WUs-AGAMOUs (AG)-KNUCKLEs (KNU) feedback loop for determinate growth of flowers. WUs expression is restricted by both direct interaction of chormatin loop formation by AG and indirectly via KNU. Initial observations showed that CLV1, CLV2, CLV3, RPK2, sTM, and LFY are maintained as single copies, while their interacting partners WUs, AG, and KNU are retained in multiple copies. This study aims to understand how duplicated genes fit into the larger regulatory network to carry out the basic regulatory mechanism. Most Brassicas, including this mesohexaploid crop temperature, are sensitive to temperature. Results will shed light on the evolutionary trajectories on duplicate fate for adaptation, domestication, and diversified morphotype growth. |
Total Budget (INR): | 38,10,304 |
Organizations involved