Research

Medical Sciences

Title :

Gentamicin-Protein interactions Identification: determination of toxicity in different cell systems

Area of research :

Medical Sciences

Focus area :

Pharmacology

Principal Investigator :

Dr. Sadhna Sharma, Profcssor, Department of Biochemistry, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, Punjab

Timeline Start Year :

2013

Timeline End Year :

2016

Contact info :

Details

Executive Summary :

There was increase in FoxO3a expression when aluminium treatment was given to differentiated PC12 cells. Further aluminium exposure resulted in increase in mRNA expression of cell cycle activators cyclin D, cyclin A and cdk4 and decrease in mRNA expression of cell cycle inhibitor p21 which indicate activation of cell cycle induces cell death in neurons. Whereas, resveratrol exposure resulted in decreased mRNA expression of cyclin D, cyclin A and cdk4 and increased mRNA expression of p21, which inhibits cell cycle and prevent cell death in neurons. Histochemical analysis also revealed that aluminium treated brain hippocampus sections has marked cell distortion and high level of degeneration in cells as compared to control rat brain. However, pretreatment of resveratrol prevented cell damage. Aluminium also showed its deleterious effects in terms of oxidative damage, cholinergic impairments and associated cognitive functions in rats whereas resveratrol protects the same. This study demonstrates that resveratrol having antioxidant and metal chelating properties may be used as therapeutic entity for ameliorating aluminium inffi neurodegeneration. So it can be concruded that resverahol can be used as protective against aluminium induced neurotoxicity.

Co-PI:

Dr. K.D.Gill, Professor, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh

Organizations involved