Medical Sciences

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Objective: Development of optical sensor for measuring homocysteine directly and selectively in the blood samples of CVD and MCI patients. Development of homocysteine specific optical kit for analysis of CVD and MCI patients’ blood samples. Gold standard validation of the developed sensors for sensitivity and specificity. Development of electrochemical sensor for Hcy measurement in biological samples. Clinical validation of the optical and electrochemical sensors with CVD and MCI patients’ samples. Shelf life as solid powder: 12 months (at 25 ?C, relative humidity ~65%) Seek for certification/ approval from competent authority.

Summary: This project proposal origin from our recent development of a cost-effective sensor that can measure Hcy directly in human blood plasma. The sensor is completely soluble in water and therefore, entire analysis can be performed in non-organic medium. Moreover, the probe can detect Hcy directly and selectively even in presence of cysteine and glutathione. This project proposal primarily aims at the direct detection and quantitative measurement of Hcy for cardiac and MCI patients. Finally, Synthesis of the sensor in larger quantities; physicochemical and photophysical studies for characterization; SOP development; and cost optimization will be perused. Electrochemical kit(s) will be delivered after clinical validation of the electrochemical sensor(s). The sensor will be integrated to the electrode surface using different approaches (electrodeposition/entrapment in a polymer). Two different types of compounds (aqueous soluble and organic soluble) that are Hcy specific will be investigated to develop electrochemical strategies. The selectivity of the proposed sensor for homocysteine at different scan rates will also be investigated to evaluate the selectivity of the proposed method. At higher scan rate, homocysteine can be oxidized easily at the electrode surface rather than low-scan rate.

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