Technologies

Agricultural Sciences

Title:

Diagnostic kit to detect Citrus tristeza virus in citrus Dip stick format and ELISA format based on Monoclonal antibodies

Area:

Agricultural Sciences

Sub Area:

Diagnostic kit

Focus Area:

Immunology

Developing Agency:

Indian Institute of Horticultural Research (IIHR), Bengaluru

Technology Readiness Index:

Technology developed

Email:

director.iihr@icar.gov.in
Website Link :https://iihr.res.in/diagnostic-kit-detect-citrus-tristeza-virus-citrus-dip-stick-format-and-elisa-format-based

Brief Description

Description :

The kit is simple to operate can be used by illiterate farmer in the field without any lab facilities as long as there is clean water. It involves crushing the leaf sample to get a few drops of sap which can be collected in a tube into which the stick coated with regents is dipped followed by dipping in detection regent and detection substrate. A positive and a negative control are included for reliability of the results. Washing of the dip stick in clean water are intermediary steps for which clean water can also be included in the kit. ELISA format is for the researcher and semi quantitative and quantitative estimations can be done

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