IN194308: An improved process for the recovery of proteins from cheesewhey

An improved process for the recovery of proteins from cheese whey by aqueous two-phase extraction which comprises; a) preparing a mixture of polyethylene glycol, di-potassium hydrogen phosphate and potassium di-hydrogen phosphate in a ratio of 10-l6:5-&:3-4, b) adding cheese whey in a ratio of 1:5-6.5 (w/w) of polyethylene glycol to the above said mixture, c) stirring the above mixture for a period of 20-40 min by using a mechanical agitated contractor, d) allowing the phases formed in the above said mixture to separate in a separating funnel for period of 5-12 hrs and separating the top protein rich phase, e) adding peg to the above said protein containing phase, mixing thoroughly and phase separating by known methods as herein described to obtain the desired fat free protein concentrated phase, and PEG phase.