Patents
A process for the preparation of nanosized hydroxyapatite, which comprises; i) mixing calcium nitrate solution of strength 0.2-0.4 M and ammonium hydroxide solution of strength ranging between 3 to 5M in a volumetric ratio of 5:1 to 7:1 by continuous stirring using a magnetic stirrer, ii) mixing the above-said mixture with bovine serum albumin solution of strength ranging between 0.05-2% in a volumetric ratio of 1:3 to 1.5:3 under nitrogen atmosphere and stirring for 20 minutes by a magnetic stirrer, iii) heating the above resultant solution at a temperature in the range of 60-75°C for a period of 18-24 hours under nitrogen atmosphere to obtain calcium load 2-4 hours into alkaline diammonium hydrogen phosphite solution of strength 0,14-0.16 M, at a temperature ranging v) washing the above-soaked protein gel with deionized water to remove ammonium nitrate formed as by-product and recovering the nanosized hydroxyapatite from the soaked protein gel by the known method as herein described.