IN199821: A process for the preparation of a vaccine against tuberculosis

M. tuberculosis was cultivated in allogeneic (AMTV) and syngeneic (SMTV) macrophages and was killed by Y-irradiation and was used as a vaccine. The AMTV in vivo will preferably be engulfed by dendritic cells (Y-irradiation causes cells to undergo apoptosis and dendritic cells engulf apoptotic cells) and will then activate the mycobacterium reactive naive T cells. Allogeneic macrophages being used for immunization. Dendritic cells are the preferred Antigen Presenting Cells (APC) for Th1 and cytotoxic T cells (CTL), Th1 and CD8+ CTL are principal cells in generating effective and protective immunity against M. tuberculosis.