Patents
A process for the removal of toxic aflatoxin B1 from coconut protein isolates which comprises;
i) isolating the protein by dispersing defatted coconut meat in waterm in the temperature range of 20 to 30°C to obtain mixture containing protein,
ii) adjusting the pH of the mixture in the range of 8-10 by adding conventional diluted alkali at a temperature in the range of 20 to 30°C,
iii) stirring the mixture for a period to 30 minutes;
iv) filtering the mixture, adjusting the pH of clarified protein extract in the range of 4 to 5 by adding conventional dilute acid,
v) separating protein isolate by vacuum evaporation from clarified protein extract,
vi) treating the said protein isolate with hydrogen peroxide having strength in the range of 0.2 to 0.5% (v/v) at a temperature in the range of 55-95°C for a period in the range of 10-40 minutes,
vii) drying the above treated protein isolate of step vi) to obtain the aflatoxin Bl free coconut protein isolate.