Life Sciences & Biotechnology
Title : | Identification of SARS-CoV-2 pseudoknot binding ligands that inhibit-1 programmed ribosomal frameshifting -1PRF |
Area of research : | Life Sciences & Biotechnology |
Focus area : | Molecular Pharmacology |
Principal Investigator : | Ms. Neha Jeena, Central University of Rajasthan, Ajmer, Rajasthan (305817) |
Timeline Start Year : | 2024 |
Timeline End Year : | 2027 |
Contact info : | neha.jeena1@gmail.com |
Details
Executive Summary : | This proposal aims to identify the SARS-CoV-2 pseudoknot binding agent that inhibits -1 programmed ribosomal frameshifting. The objective is divided into four groups: virtual screening of the library database on the PDB ID 7LYJ structure of SARS-CoV-2 pseudoknot, biological screening of the RNA library against dual-luciferase reporter system assay, antiviral activity of HITS from dual luciferase frameshift assay, validation of RNA binding using surface plasma resonance studies, and cytotoxicity and in-vitro ADME profiling of active compounds. The project focuses on developing a robust virtual screening model to identify RNA binding compounds having affinity for SARS-CoV-2 pseudoknot, determining if inhibitors have the potential to inhibit the expression of pseudoknot control firefly luciferase, and understanding how the discovery of effective pseudoknot-targeting ligands impact the development of antiviral therapies against SARS-CoV-2. |
Total Budget (INR): | 19,01,880 |
Organizations involved