Research

Life Sciences & Biotechnology

Title :

A scalable system for the production of human induced pluripotent stem cell derived functional retinal pigmented epithelial cells

Area of research :

Life Sciences & Biotechnology

Focus area :

Stem Cell-Based Therapies

Principal Investigator :

Dr. Anwar Azad Palakkan, Aravind Medical Research Foundation, Tamil Nadu

Timeline Start Year :

2023

Timeline End Year :

2026

Contact info :

Details

Executive Summary :

Age-related macular degeneration (AMD), which occurs due to the degeneration of Retinal Pigmented Epithelial cells (RPE) is the third most common cause of blindness. Currently there are no treatments to modify the progression of AMD, and the patient eventually becomes blind. Most effective way to treat this disease is to replace the damaged cells. In recent years pluripotent stem cell technology has evolved much and there is a great focus on using iPSC-derived RPE (iRPE) for therapy. Instead of generating iPSC and differentiating them to RPEfor each patient, a more economical and practical approach is the off-shelf availability of iRPE's of different homozygous HLA type (this will increase number of matching recipients). To achieve this a scalable method to bulk produce iRPE need to be developed. As the quality of iRPE can vary, a standard assay to estimate the quality of such cells also need to be developed. The proposed project aim to address these issues. Our proposed work will help to establish a robust scalable method to bulk produce functionally competent iRPE and to develop a simple tool to easily evaluate their quality. A method to generate iRPE cell sheet will also be established.

Co-PI:

Dr. GowriPriya Chidambaranathan, Aravind Medical Research Foundation, Madurai, Tamil Nadu-625020

Total Budget (INR):

47,63,593

Organizations involved