Life Sciences & Biotechnology
Title : | Characterization of a novel alpha or beta Hydrolase protein in Plasmodium falciparum and understanding its role in Artemisinin Resistance |
Area of research : | Life Sciences & Biotechnology |
Focus area : | Molecular Biology |
Principal Investigator : | Dr. Krishanpal Karmodiya, Indian Institute of science Education and Research (IIsER), Pune, Maharashtra |
Timeline Start Year : | 2024 |
Timeline End Year : | 2027 |
Contact info : | krish@iiserpune.ac.in |
Details
Executive Summary : | Malaria remains a significant issue in developing countries, with Plasmodium falciparum being the primary cause of malaria-related deaths. Artemisinin resistance has been reported in southeast Asia and India, with mutations in the Kelch13 gene identified as a molecular marker. K13 mutant parasites have reduced hemoglobin endocytosis, slow growth, damaged cellular components, and heightened stress response. The enhanced lipid metabolism and intracellular trafficking of PI3P-rich vesicles contribute to artemisinin resistance. Recent studies have reported K13-independent artemisinin resistance in central India and Africa. A study using genomic sNP datasets from 2,517 isolates from 15 countries identified mutations in a novel putative gene named alpha-ß hydrolase. The study aims to understand the role of alpha or beta hydrolase in regulating artemisinin sensitivity in P. falciparum, determining its catalytic activity and gene knockout studies. |
Total Budget (INR): | 46,37,080 |
Organizations involved