Life Sciences & Biotechnology
Title : | Understanding the protective effect of chlorogenic acid in rat embryonic neural stem cells prenatally exposed to air particulate matter: A transcriptome-based approach |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Abhishek Kumar Singh, Manipal Academy Of Higher Education, Karnataka |
Timeline Start Year : | 2024 |
Timeline End Year : | 2027 |
Contact info : | aks84m@gmail.com |
Equipments : | PM Sampler and DustTrak aerosol monitor
Biosafety cabinet
GPU Workstation and Server |
Details
Executive Summary : | Air pollution is the second largest risk factor for global non-communicable diseases, with particulate matter (PM) being linked to higher risks of neurodevelopmental and neurodegenerative disorders, poor mental health, and cognitive defects. The neurogenesis process during embryonic development is particularly vulnerable to PM exposure due to the poorly developed blood brain barrier and placental barrier. The brain development process involves neural stem cells (NSCs) proliferation, differentiation, maturation, neuritogenesis, and synaptogenesis. However, the adverse effect of PM on neurogenesis during embryonic development is not well understood, and there is no information on transcriptional responses to PM exposure in NSCs proliferation and differentiation. This field of research is still in its infancy, and efforts are needed to clarify the precise mechanisms by which PM affects neurodevelopmental events. The study aims to investigate the adverse effect of prenatal exposure of nano-sized air particulate matter on stemness, proliferation, and differentiation of NSCs through transcriptomics analysis and molecular mechanisms. The identified genes merit further mechanistic studies for therapeutic intervention development. The study also aims to investigate the protective effects of chlorogenic acid (CGA) against PM-induced detrimental effects in embryonic neural stem cells. |
Co-PI: | Prof. Subhrajit Biswas, Amity University, Noida, Uttar pradesh-201313, Dr. Vijay Kumar, Amity University, Noida, Uttar pradesh-201313, Dr. Raviraja NS, Manipal Academy Of Higher Education, Karnataka-576104 |
Total Budget (INR): | 54,52,120 |
Organizations involved