Life Sciences & Biotechnology
Title : | Elucidating the Interplay between Ligand-Binding and DNA-Recognition in the Transcription Regulator CytR |
Area of research : | Life Sciences & Biotechnology |
Focus area : | Biotechnology, Genetic Engineering |
Principal Investigator : | Dr. Athi Narayanan Naganathan,
Associate Professor,
Indian Institute of Technology, Madras |
Timeline Start Year : | 2023 |
Timeline End Year : | 2025 |
Contact info : | athi@iitm.ac.in |
Details
Executive Summary : | CytR, a transcription regulator, plays a crucial role in E. coli's energy metabolism, particularly under stressed conditions. It regulates gene expression, maltose utilization, and heat-shock response. CytR's multi-functionality is due to its interaction with multiple promoter regions, including the uniquely disordered DNA binding domain (DBD). However, little is known about CytR's structural-mechanistic features, such as the cytidine binding signal and its binding affinity. The binding affinity is significant between two sub-domains of the LBD, suggesting a pre-equilibrium between open and closed conformations. Mutations can be engineered into CytR LBD to alter binding affinity. |
Total Budget (INR): | 49,07,000 |
Organizations involved
