Executive Summary : | The study proposes a novel method for local pH measurements using microrobots. This method involves designing rod-shaped, light-driven microrobots that can swim through the medium and be controlled by light or external magnetic fields. These microrobots will be coated with a pH responsive fluorescent dye, and after guided swimming, they will be aimed to measure the local pH around target locations. The experiment will be performed under an optical inverted fluorescence microscope connected to a spectrophotometer and light source. Experiments will be conducted to optimize experimental parameters for better control of the swimming and spatial position of microrobots and pH measurements. This project provides a novel platform for on-demand detection of local pH using pH-responsive dye functionalized microrobots. Unlike traditional methods where the probe must be in constant contact with the sample area, these microrobots can be activated on-demand to measure the local pH of the sample and can be taken back to the initial position once the measurement is done. The proposed microrobot-based pH sensing method is simple, clean, and fast, offering a new approach to measuring pH in various applications such as electrochemistry, extracellular pH measurements, drug effects investigation, and chemical reaction monitoring. The study aims to address the practical limitations of existing methods and develop a fast, simple, and reliable method for local pH measurements. |