Life Sciences & Biotechnology
Title : | Development of biodegradable and biocompatible hair keratin-cellulose acetate based nanofibres scaffold as a potential candidate for tissue engineering |
Area of research : | Life Sciences & Biotechnology |
Focus area : | Biomaterials, Tissue Engineering |
Principal Investigator : | Dr. Keshaw Ram Aadil, Govt. Digvijai College, Chhattisgarh |
Timeline Start Year : | 2023 |
Timeline End Year : | 2026 |
Contact info : | kaadil7@gmail.com |
Details
Executive Summary : | Researchers have gained huge interest in tissue engineering, as efforts are made to design and construct micro/nano scaffolds based on artificial environments that mimic the cellular niche. The foremost challenge in this field is to thoroughly mimic the properties and hierarchical construction of the extracellular matrices of the inherent cells. Biomaterials mainly proteins and polysaccharides are the richest renewable biopolymers in nature and have increased attention, due to their non-toxic, biocompatibility, and cell adhesion properties along with optimum physicochemical, and thermal properties. Currently, electrospun nanofiber acts as one of the noteworthy inventions of nanotechnology. Due to their high surface-to-volume ratio, nanofiber can increase drug absorption and mass transport properties. Electrospun nanofiber scaffolds have developed diverse applications in biomedical including tissue engineering, wound dressing, therapeutics drug delivery system, and enzyme immobilization. Hence, the objective of the present research proposal is to design, fabricate, characterize and study the potential of hair keratin-cellulose acetate nanofiber scaffolds for tissue engineering applications. Keratin-cellulose (KC) acetate based nanofibers will be fabricated using the electrospinning technique and their potential as scaffolds for tissue engineering will be studied. Keratin, extracted from human hair will be blended with cellulose acetate in an aqueous medium. Extensive morphological and chemical characterization of the fabricated nanofiber scaffolds will be performed using sophisticated techniques. Moreover, the in-vitro cell culture study of scaffolds will be tested on human keratinocytes and dermal fibroblast cell lines. The proposed work will reveal the potential of keratin-cellulose acetate based nanofiber scaffolds as tissue engineering grafts. The cell culture study will be demonstrated the potential of scaffolds for skin tissue engineering and it can also be a favorable material for different tissue engineering applications. Further, the use of natural biomaterials hair keratin for the fabrication of nanofibers scaffolds will be advantageous over the previously used non-biodegradable synthetic materials. The usage of Normal Human Dermal Fibroblasts and primary keratinocytes seeded tissue grafts, as proposed, may accelerate the rate of tissue regeneration and henceforth would reduce the healing time. The specific morphology of nanofiber scaffolds will mimic the extracellular matrix components and support the tissue cell's growth and proliferation. Tissue grafts loaded with biochemical cues/nutritional components would have great potential and applications as smart and bioactive tissue grafts for various tissue engineering applications. Further, the study would contribute to good quality and high-impact research work in the form of articles, and patents and also contribute to maintaining sustainability and a circular economy. |
Total Budget (INR): | 28,93,900 |
Organizations involved