Research

Life Sciences & Biotechnology

Title :

Mechanism of chiral proofreading of DTD2 from archaea and plants

Area of research :

Life Sciences & Biotechnology

Focus area :

Biochemistry, Structural Biology

Principal Investigator :

Dr. Rajan Sankaranarayanan, CSIR- Centre For Cellular And Molecular Biology (CSIR–CCMB), Hyderabad, Telangana

Timeline Start Year :

2024

Timeline End Year :

2027

Contact info :

Details

Executive Summary :

Land plants have developed metabolic pathways that involve the production of reactive aldehydes and their detoxification to survive harsh environments. A chiral proofreader, DTD2, plays a critical role in detoxifying anaerobic stress-induced acetaldehyde modification of D-aminoacyl-tRNA. This study aims to identify DTD2's role in protecting plants from metabolically generated aldehydes, elucidating its mechanism of chiral selectivity and adduct activity, and identifying key residues involved in its biochemical activities. Understanding molecular determinants providing aldehyde modification specificity in DTD2 can help engineer DTD2 protein for a broader range of protection from various cellular metabolites.

Total Budget (INR):

68,21,020

Organizations involved