Life Sciences & Biotechnology
Title : | Mitochondrial respiration in setaria italica, a C4 millet, and its interplay with photosynthetic components under high light stress |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Prof. Kollipara P.M.s.V. Padmasree, University Of Hyderabad, Telangana |
Timeline Start Year : | 2024 |
Timeline End Year : | 2027 |
Contact info : | kpssl@uohyd.ac.in |
Equipments : | LED light source
Plant Efficiency Analzer (Chlorophyll Fluorescence meter) |
Details
Executive Summary : | Our research group has been working on the essentiality of mitochondrial oxidative electron transport for optimal photosynthesis for the past three decades using mesophyll cell protoplasts (MCP) of Pisum sativum and Arabidopsis thaliana as the model systems. Biochemically, photosynthesis and respiration are complementary to each other, occurring in two autonomous organelles, namely chloroplasts and mitochondria. Now, it is clearly established in C3 plants that these organelles are interdependent and benefit each other (Raghavendra and Padmasree, 2003). In C4 plants, the interactions between chloroplasts and mitochondria are known in NAD-Malic enzyme (NAD-ME) type and phosphoenolpyruvate carboxykinase type (PEP-CK) type C4 plants (Fan et al., 2022). But, the information on such interactions between chloroplasts and mitochondria of NADP-malic enzyme (NADP-ME) type C4 plants is not revealed or is very meager. The present study aims to fill the gaps in understanding the various functions of COX and AOX pathways of mitochondrial electron transport chain (mETC) in benefiting different photosynthetic components such as PsII, PsI, and Calvin-Benson cycle in C4 NADP-ME type plants. Moreover, two types of cells MCP and bundle sheath strands (Bss) participate in C4 photosynthesis. Therefore, it would be interesting to understand if the mETC of MCP or Bss of NADP-ME C4 plants plays a role in mitigating stress responses by adjusting cellular redox and reactive oxygen species (ROs). A model was shown to project the gap and the importance of the proposed project in the context of the current status of metabolic interactions between organelles, MCP and bundle sheath cells in NADP-ME-type plants. Also, we have chosen drought and salt-sensitive (IC41) and tolerant (IC4) varieties of s. italica to study the effect of high light stress in the present study. Furthermore, the generation of knockout mutants for COX and AOX pathways would establish the relative importance of these mETC pathways in sustaining photosynthesis under high light. The s. italica is one of the millets recognized for human consumption based on the merit of possessing several nutritious molecules. The present study using knockout mutants of COX and AOX from s. italica would unravel the important respiratory component that could be targeted for achieving sustainable agricultural food production in C4 NADP-ME type plants. As the year â2023' is recognized as the "International Year of Millets," the objectives proposed in the present study are in agreement with the motto of Govt. of India. |
Co-PI: | Dr. Muthamilarasan M, University Of Hyderabad, Telangana-500046, Prof. Agepati s Raghavendra, University Of Hyderabad, Telangana-500046 |
Total Budget (INR): | 52,09,600 |
Organizations involved