Life Sciences & Biotechnology
Title : | Multipronged studies on persistence and drug resistance in mycobacteria |
Area of research : | Life Sciences & Biotechnology |
Focus area : | Persistence and drug resistance in mycobacteria |
Principal Investigator : | Dr B N Singh, Scientist, CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow |
Timeline Start Year : | 2020 |
Timeline End Year : | 2025 |
Contact info : | director@cdri.res.in |
Details
Executive Summary : | Objective: Using sigF mutant model identify the set of genes that are differentially expressed at different stages of biofilm development; Identify the factors responsible for biofilm maturation and inducing biofilm dispersion in M. tuberculosis; Identify the common bacterial players involved in dispersion under different conditions; Development of recombinant mycobacterial strains as model for biofilm mediated screening; Compare virulence of dispersed bacilli to planktonic bacilli; Delineate a strategy to induce concomitant biofilm dispersion and anti-mycobacterial treatment; Gene expression and metabolite profiling of persister cells; Identification of core metabolic enzymes in persistence and development of recombinants; Delineation of host immune response to infection by recombinant and wild-type Mtb; Development of in silica model, screening of inhibitor libraries, identification of new scaffolds and rationalized screening; Structural studies and Functional characterization of c-di-NMP metabolizing enzymes, Mtb persistence target identified by TB consortium. |
Organizations involved