Life Sciences & Biotechnology
Title : | Production of Isomaltooligosaccharides (IMO) using indigenous transglucosidase from primary and secondary starch sources |
Area of research : | Agricultural Sciences, Life Sciences & Biotechnology |
Focus area : | Production of Isomaltooligosaccharides |
Principal Investigator : | Dr M.V.R.K Sarma, Scientist, CSIR-Central Food Technological Research Institute (CSIR-CFTRI), Lucknow |
Timeline Start Year : | 2020 |
Timeline End Year : | 2022 |
Contact info : | director@cftri.res.in |
Details
Executive Summary : | Objective: Optimize fermentation process at 100 Liters for a-glucosidase production using fungal isolate; Standardization of simultaneous saccharification and transglucosylation process for IMO production using starch at 50-100 Liters Summary: Under FSSAI’s Nutraceuticals Regulations (2016), Isomalto-oligosaccharides (IMO) are considered as prebiotic compounds. From our earlier studies at CSIR-CFTRI, a fungal strain producing tranglucosylating a-glucosidase has been isolated from soil which was used for synthesis of IMO using enzyme-substrate (maltose) reaction. Comprehensive studies have been conducted on characterization of the organism, optimal cultivation for a-glucosidase in bench scale bioreactor (10 L) and optimization of IMO production in a one pot procedure (0.5 L) a . Presently, there is no Indian company for either IMO or transglucosylating a-glucosidase production. The present work intends to continue potential results obtained from earlier studies and demonstrate the viability of IMO production commercially using starch-derived wastes. |
Organizations involved