Life Sciences & Biotechnology
Title : | Ras Effector RASSF10 locks the metabolic reprogramming mechanism in tumour cells |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Prof. Sundarasamy Mahalingam, Indian Institute Of Technology (IIT) Madras, Tamil Nadu |
Timeline Start Year : | 2023 |
Timeline End Year : | 2026 |
Contact info : | sundarmahalingam1964@gmail.com |
Equipments : | Western Blot apparatus: PAGE Running and transfer apparatus
Thermal Cycler |
Details
Executive Summary : | Tumour development is a complex process with various biological capabilities acquired at each stage. Deregulation in developmental pathways, including RAS, alters cell fate decisions. RASSF10, a member of novel nonenzymatic RAS effectors, is downregulated predominantly through hypermethylation in over 60% of cancers. RASSF10 inhibits cell proliferation, survival, and migration, but its mechanism is yet to be elucidated. High throughput assays have identified nucleolar Guanine nucleotide binding protein Like 3 Like (GNL3L) and Inosine-5?-monophosphate dehydrogenase-2 (IMPDH2) as potential targets of RASSF10. Results from RT-qPCR analysis reveal that RASSF10 downregulates both GNL3L and IMPDH2 expression. GNL3L plays a critical role in normal cellular biology, while IMPDH2 is a critical enzyme for de novo guanine nucleotide biosynthesis. Both GNL3L and IMPDH2 are overexpressed in many solid tumors and negatively correlate with patient survival. The hypothesis is that RASSF10 may control metabolic reprogramming in cancer cells by modulating the expression levels of GNL3L and IMPDH2. The study proposes to characterize the mechanism(s) by which RASSF10 regulates nucleolar function and metabolic programming to control cell proliferation during tumorigenesis and to identify the mechanism by which RASSF10 suppresses GNL3L and IMPDH2 expression in cancers to control nucleolar hypertrophy. |
Total Budget (INR): | 76,70,237 |
Organizations involved