Description : | The Micropropagation protocol for pineapple developed at BARC includes tissue culture propagation using shoot tips as well as dormant axillary buds from pineapple crowns on a defined nutrient media containing specific plant growth regulators. The steps include sterilized shoot tips obtained from the parent pineapple plant, shooting and rooting in the test tube, primary hardening in the laboratory, secondary hardening in the nursery and plating in the field. One of the main advantages is the production of disease free uniform age and size planting material which can be made available throughout the year. |