Patents
A process for the preparation of stabilized biocatalyst containing D-amino acid oxidase activity which comprises: a) Cultivating Trrgnopsis variabilis in a synthetic or a complex nutrient medium such as herein described. b) Harvesting the cells and washing them with 50 Mm Tris buffer of pH 2.0 c) Permeabilizing the enzymatically active nonviable whole cells or cell homogenate or cell fragments by treatment with 2% toluene, d) Suspending the permeabilized cells in polyamine such as polyethyleneimine of molecular wt. 1000-50,000 with concentration of polythyleneimine in the microbial cell suspension is o.5-5%, e) Treating the cell suspension with 0.05-2% of 1,4-butanediol diglycidyl ether at a temperature in the range of 4 to 30 °C for 24 to 72 hrs.. f) Treating with amino compound containing primary amine group such as herein described to obtain stabilized biocatalyst. g) Optionally converting stabilized biocatalyst to suitable form such as beads using agar-agar or agarose.