Patents
The invention relates to a process for the development of a highly efficient solid state matrix by the activation of acrylate based polymer resin having specialized functional groups with 1,1-Carbonyl diimidazole for immobilizing biologically active macromolecules such as oxidases, in particular plant oxidases and the most preferred being tea polyphenol oxidase through indirect covalent bonding/cross linking on such activated polymer resin support, are thermally stable, gives very high number of turnovers in vitro ("n" times) with tea substrate forming exclusive product. Theaflavins without any loss of biological activity and leaving the product remaining in vitro without adherence to matrix rendering the matrix safe towards product poisoning and subsequent partial or complete loss of biological activity of the matrix bound enzyme system and thus well adapted to and well suited bioreactors based on such systems. It is unique with respect to its recyclability of otherwise uneconomical tea substrates such as seed and flower substrates into theaflavins both with respect to crude substrate or purified ones.