Patents
The multi-layered scaffold could be applicable for tissue engineering/regeneration, drug delivery and wound dressing. The process may be used for phase-wise delivery of the growth factors which would be suitable for the studies of developmental biology. The novelty disclosed is the various ratios of the therapeutics/pharmaceuticals like antimicrobial agents, analgesics, antioxidants, antiallergic, growth factors and/or other signaling molecules like Lysophosphatidic acid (LPA), either in free form or microparticles encapsulated or combination of both can be taken to formulate the multi-layered porous scaffold with different concentrations of natural or chemically modified collagen. Microparticles comprise minimum of two types of polymers e.g. gelatin, chitosan, alginate, casein, PLGA, PCL and multi-layer scaffold contains at least two layers out of which one may be thin film layer without therapeutics, may act as a protective layer, which is desirable for wound dressing. The multi-layered porous scaffold prepared by homogenous casting of collagen solution on non-sticky substratum and allowed for semi-dry, similarly, the subsequent collagen layers were prepared and followed by freeze-dry or air-dry. In case of air-dry, the collagen solution was homogenized with surfactants like triton X-100. Cross-linking agents e.g. glutaraldehyde can be used to prolong the release of therapeutics. The product, therapeutics may be antibiotic(s) or analgesic(s) of growth factor(s) or any combinations for immediate and sustained/controlled delivery.