Life Sciences & Biotechnology
Title : | Characterisation of the protective role of omega 3 fatty acid against metal mixture induced neuro- developmental damage |
Area of research : | Life Sciences & Biotechnology |
Focus area : | Nutrition |
Principal Investigator : | Dr. Sanghmitra Bandyopadhyay, Indian Institute of Toxicology Research (IITR), Lucknow, Uttar Pradesh 226001 |
Timeline Start Year : | 2010 |
Timeline End Year : | 2012 |
Contact info : | sanghmitra@iitr.res.in;
mailesisecretariat@gmail.com |
Details
Executive Summary : | The omega 3 fatty acids (?3FAs) alleviate apoptosis in the neuro leading to a protection to the injured brain. However, their anti-apoptotic activity in astrocytes is obscure. IIcre, we hypothesized an an apoptotic role of w3FAs in the damaged developing astrocytes that may involve modulation in the levels of glial fibrillary acidic protein (GFAI peroxisome proliferator-activated receptors (PPAR), and the fatty acid binding 7 (FABP7), a strong binder of the w3I'As. The w3KAs we supplemented as a mixture of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) to pregnant and lactating rats. The apoptosis in t rat astrocytes was then induced by a mixture of the metals, Pb, Cd and As (MM, metal mixture). The ?3FAs prevented the MM-induc attenuation in the GFAP levels, PPAR (alpha, delta and gamma and FABP7 in the brain tissues. The MM raised the levc of the PPARs in the GFAP-immunoreactive astrocytes, and omega 3 fatty acid prevented the increase. The MM up-regulated the lev« of FABP-7 in the astrocytes, and ?3 fatty acid maintained the up-regulated status, probably because of the binding of the fatty acids w: the FABP7. The MM promoted the translocation of PPAR alpha, delta and gamma to the nucleus and omega 3 fatty acid prevented. This, therefore, indicates that translocation of PPARs to nucleus damages astrocytes, and ?3 fatty acid prevents translocation and prevents astrocyte damage. |
Co-PI: | Prof. M. M. Godbole, Head, Endocrinology, Sanjay Gandhj Post Graduate Institute of Medic Sciences (SGPGIMS), Lucknow, Uttar Pradesh 226014 |
Total Budget (INR): | 4,90,000 |
Achievements : | Research scientist/fellow 01 |
Publications : | 3 |
Outcome/Output: | The data, for the first time, demonstrate that ?3 fatty acid protects the brain from MM-induced stress and thus has prophylact role during brain development. |
Organizations involved