Life Sciences & Biotechnology
Title : | Characterisation of Wnt signaling pathway as a potential therapeutic target for Ligamentum flavum hypertrophy in Lumbar canal stenosis |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. G sudhir, sri Ramachandra Institute Of Higher Education And Research, Tamil Nadu |
Timeline Start Year : | 2023 |
Timeline End Year : | 2026 |
Contact info : | sudhir_axon@yahoo.co.in |
Equipments : | Tissue Homogeniser
Deep Freezer
Kerrison Rongeurs |
Details
Executive Summary : | Lumbar canal stenosis (LCs), a degenerative spine pathology in the elderly, is a leading cause of morbidity. Ligamentum flavum (LF) hypertrophy is crucial in the pathophysiology of LCs, but its molecular mechanism remains unclear. The current standard of treatment for LCs is surgical, but it can lead to complications. Few studies have documented that Wnt signaling plays a vital role in LF hypertrophy. The study hypothesizes that the Wnt canonical pathway plays a significant role in the development of LF hypertrophy and fibrosis. Patients with LCs and nonhypertrophied LFs from patients with lumbar disc herniation will be analyzed for Wnt3a, β-catenin, and vascular endothelial growth factor A (VEGF-A) by immunohistochemistry. The effect of Wnt antagonist and Wnt3a on cell morphology, proliferation, and migration will be examined using a primary cell culture of human hypertrophied or nonhypertrophied LF. The analysis of Wnt-βcatenin signaling in LF tissues and correlation with MRI could help develop diagnostic and prognostic biomarkers. |
Co-PI: | Dr. Lakshmirevathi Perumalsamy, sri Ramachandra Institute Of Higher Education And Research, Tamil Nadu-600116, Prof. sandhya sundaram, sri Ramachandra Institute Of Higher Education And Research, Tamil Nadu-600116 |
Total Budget (INR): | 62,45,613 |
Organizations involved