Life Sciences & Biotechnology
Title : | Characterizing the Mechanistic Insights and Clinical Relevance of Fusobacterium in Tongue Cancer |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Amit Dutt, Tata Memorial Centre (Hospital), Maharashtra |
Timeline Start Year : | 2023 |
Timeline End Year : | 2026 |
Contact info : | adutt@actrec.gov.in |
Details
Executive Summary : | The Human Microbiome Project has identified 48 microbial habitats in the human body, maintaining balanced symbiotic/commensal relationships. A shift in this balance, known as a 'dysbiosis', can lead to disease, with chronic inflammation often linked to cancer initiation and progression. The role of the microbiome in human cancer has become a focus of research. A recent study identified a significant prevalence of Fusobacterium in tongue cancer samples, similar to its occurrence in colorectal tumors. Fusobacterium is mutually exclusive to HPV infection and is characterized by overexpression of miRNAs associated with inflammation, elevated innate immune cell fraction, and nodal metastases. The study proposes to extend the findings by examining the functional validity and relevance of the Fusobacterium/tongue cancer association, the influence of Fusobacterium on tongue cancer growth, and the effect of Fusobacterium on the immune microenvironment. This will involve studying the impact of the bacterial infection on the proliferation, invasion, migration, and other characteristics of tongue tumor cells. The study will also identify host-induced molecular correlates in tongue cancer cells in response to Fusobacterium infection. Lastly, the study plans to establish a tongue cancer orthotopic mouse model and develop the first patient-derived-xenograft (PDX) model and PDX-derived primograft cultures in tongue cancer. This will investigate the clinical utility of Metronidazole, a broad spectrum microbial inhibitor, in impeding the growth of tongue tumors in PDX mice model and PDX-derive primograft cellular culture. |
Co-PI: | Dr. Anirban Banerjee, Indian Institute Of Technology (IIT) Bombay, Mumbai, Maharashtra-400076, Prof. sudhir V Nair, Tata Memorial Centre (Hospital), Mumbai, Maharashtra-400012, Prof. Tapasya srivastava, University Of Delhi, south Campus, New Delhi-110021 |
Total Budget (INR): | 67,10,347 |
Organizations involved