Executive Summary : | A total of 95 samples were analysed for various in vitro studies and molecular analysis in our study. Sixty-four isolates were revived and cultured out of which 45 isolates were successfully adapted and further processed were also carried out. Gametocyte production was studied in these 45 isolates and successful production was seen in 19 isolates. The samples were successfully analyzed for msp1 msp2 and for glurp loci for classification of the allelic families prevalent in the isolates. The IC50 values for CQ have shown a remarkable reduction on comparison with the previous available data, whereas a slight increase in the IC50 values for AS and a relatively high values for SD and PYR were observed in the samples indicating the rise of drug tolerance for these three drugs in the parasite population. The samples were initially screened for Pfs25 and Pfg377 gametocyte genes where the expression levels ranged from 0.11-9.12 and 0.08-30.37 fold for Pfs25 and Pfg377 gene on comparison with the reference isolate NF54 by RT-PCR. The allele typing of Pfg377 gene in the isolates revealed four different genotypes viz Type A, B, C and D varying in sizes, between 280 and 400 bp with the most common allele being 350 bp. The cryopreserved and adapted samples of the same isolates showed difference in their genotypes on allele typing indicating the presence of multiple clones in the same sample on sequence comparisons. The isolates were also analysed for the presence of mutant alleles in drug resistance genes viz: crt-K76, mdr-1 N86 and Y86, dhfr-C59, S108 and dhps-K540. |
Co-PI: | Dr. S. K. Gosh, Scientist, National Institute of malaria Research (NIMR), New Delhi, Dr. Anup Anvikar, Scientist, National Institute of malaria Research (NIMR), New Delhi, Dr. Ruchi Singh, Scientist, National Institute of malaria Research (NIMR), New Delhi, Dr. Jagdish Chandra, Professor, Lady Hardinge Medical College (LHMC), New Delhi, Dr. Virender Kumar, Professor, Lady Hardinge Medical College (LHMC), New Delhi, Dr. Sukla Biswas, Scientist, National Institute of malaria Research (NIMR), New Delhi |