Life Sciences & Biotechnology
Title : | Investigating the roles of sET26 and sET27 in the protozoan parasite Leishmania donovani |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. swati saha, University Of Delhi, south Campus, Delhi |
Timeline Start Year : | 2024 |
Timeline End Year : | 2027 |
Contact info : | ss5gp@yahoo.co.in |
Equipments : | Liquid nitrogen cell stock freezer
Refrigerated microcentrifuge and accessories
-80 freezer
Comet assay Electrophoresis set up
Inverted microscope with camera |
Details
Executive Summary : | sET domain proteins are typically lysine methyltransferases that mono-, di- or tri-methylate the epsilon amino group of lysine residues using s-adenosyl methionine as the methyl group donor. Originally identified as histone methyltransferases, subsequent studies revealed that these proteins target non-histone substrates as well, including proteins involved in transcription, DNA repair, DNA replication, and cell cycle control. sET proteins have been extensively investigated in yeast and human cells, but their functions in trypanosomatids remain poorly understood. To date, there is no information on the functional roles of any sET proteins in trypanosomatids, although a recent study has identified the subcellular localization of the 30 Trypanosoma brucei sET proteins. Of the 8 nuclear T. brucei sET proteins, only two were found to localize to chromatin: sET26 and sET27. The Leishmania donovani sET proteins are highly conserved with the Trypanosoma sET proteins, and thus there is a high probability of their functional roles being conserved as well. On the basis of the preliminary localization findings in T. brucei, the proposal being put forward here aims to examine the roles of sET26 and sET27 of Leishmania donovani. Using the approach of creating genomic knockouts followed by analysis of the resultant phenotypes we aim to understand the role of sET26 and sET27 in regulating Leishmania cellular processes. Considering that TbsET26 and TbsET27 localize to chromatin it is expected that LdsET26 and LdsET27 would play one or more roles in DNA replication / repair /transcription. These aspects will be examined in knockout lines. The importance of these proteins in modulating parasite survival within host cells, if any, will also be examined. |
Total Budget (INR): | 68,89,401 |
Organizations involved