Life Sciences & Biotechnology
Title : | Multi-omics network analysis to decipher miRNA-TF-gene interaction in regulation of flavonoid and carotenoid biosynthetic pathways in Chakhao cultivars of aromatic black rice from North-East India. |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Upasna Chettry, Royal Global University, Guwahati, Assam |
Timeline Start Year : | 2024 |
Timeline End Year : | 2027 |
Contact info : | upasna.chettri485@gmail.com |
Equipments : | PCR |
Details
Executive Summary : | Plant secondary metabolites, including terpenes, phenols, flavonoids, anthocyanins, and carotenoids, are a significant group of phytochemicals with potential as drugs and antioxidants. However, understanding the underlying regulatory controls determining the differential partition, transport, and accumulation of these secondary metabolites is still in its early stages. Knowledge of the regulation of flavonoids by transcription factors and carotenoids by light transcription factors is limited, and the existence of a co-regulatory network/crosstalk of miRNA-gene-TF synthesising a specific specialized metabolite is unknown. Painted rice, a staple in southeast Asia, is gaining popularity due to its presence of anthocyanins, proanthocyanidins, phenolics, γ-oryzanols, and carotenoids. However, the mechanism by which these varieties accumulate higher levels of these bioactive molecules is not fully understood. Previous research has determined the genetic and transcriptional regulators governing carotenoid metabolism in rice, and the differential expression patterns of key regulatory genes and the TF involved in the MBW complex of the flavonoid biosynthetic pathway in different colored rice varieties. The project aims to holistically decipher the metabolites accumulating in the rice system varying in the perICARp color, followed by miRNA sequencing and degradome sequencing to specifically identify the miRNA targeting the gene/TF regulating gene expression of flavanoids and carotenoids in the rice system. Unveiling these regulators in the rice system could open up new dimensions in rice breeding programs. |
Total Budget (INR): | 23,91,380 |
Organizations involved